Clin Case Rep Int | Volume 7, Issue 1 | Research Article | Open Access

The Influence of Paternal Age on Semen Parameters and Pregnancy Outcome Following Intrauterine Insemination

Jin NM1,2*, Ahmad MF3, Azmi R2 and Abdul Karim AK3

1Department of Obstetrics and Gynecology, University Technology MARA (UiTM), Malaysia
2Department of Obstetrics and Gynecology, Hospital Al-Sultan Abdullah UiTM, Puncak Alam Campus, Malaysia
3Department of Obstetrics and Gynecology, Universiti Kebangsaan Malaysia, Malaysia

*Correspondance to: Norazilah Mat Jin 

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Introduction: Male factors contribute for 25% to 40% of infertility cases, and semen quality has been commonly regarded as a measure of male fecundity. There is reported significant negative association of semen volume, total sperm count, sperm motility, and morphology with advanced male partner’s age. Therefore, combination of the sperm preparation and assisted reproductive treatment were used for a better chance of pregnancy. This study was aimed to evaluate the influence of age on the sperm quality and pregnancy outcome in couple with subfertility undergoing Intrauterine Insemination (IUI). Material and Methods: This retrospective cross-sectional study investigating 181 cases of IUI cycles from reproductive clinic of two public universities from January 2019 to December 2020. They were categorized into study group (male partner’s age 40 years and above) and control group (male partner’s age less than 40 years old). Sperm parameter reports were extracted from the patients’ records. Clinical pregnancy was the criteria for the IUI success. Results: The mean age of male and female partners were similar in both groups. Sperm parameters showed an improvement after density gradient centrifugation sperm preparation with significant results in grade 1 and grade 3 sperm motility. There was no significant different in the clinical pregnancy between the groups. Conclusion: There is an improvement in sperm parameters after density gradient centrifugation and the male age is not significantly affect the pregnancy outcome following IUI. Further study with larger sample is needed to confirm these findings.


Density gradient centrifugation; Insemination; Paternal age; Pregnancy outcome; Semen analysis


Jin NM, Ahmad MF, Azmi R, Abdul Karim AK. The Influence of Paternal Age on Semen Parameters and Pregnancy Outcome Following Intrauterine Insemination. Clin Case Rep Int. 2023; 7: 1463.

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